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  • The 16th European Microscopy Congress 2016

    Structure of filamentous alternanthera mosaic virus and mechanism of its activation.

    Evgeniya Pechnikova (1, 2), Tatiana Stanishneva-Konovalova (3), Ekaterina Petrova (3), Anton Sedov (4), Alexandre Vasiliev (2), Olga Karpova (3), Olga Sokolova (3)

    1. Laboratory of electron microscopy, Shubnikov Institute of Crystallography of the Russian Academy of Sciences, Moscow, Russie 2. NBICS Center, National Research Centre “Kurchatov Institute”, Moscow, Russie 3. Faculty of Biology, Lomonosov Moscow State University, Moscow, Russie 4. Laboratory of molecular bioengineering, Shemyakin and Ovchinnikov Institute of Bioorganic chemistry of the Russian Academy of Sciences, Moscow, Russie

    Alternanthera mosaic virus (AltMV) is a filamentous plant virus belonging to the genus Potexvirus. The virus contains a single-strand RNA and coat protein (CP) that…
  • The 16th European Microscopy Congress 2016

    Switching mechanism of DNA replication fork complex revealed by single particle analysis

    Kouta Mayanagi (1, 2), Sonoko Ishino (3), Mika Takafuji (3), Kaoru Mitsuoka (4), Tsuyoshi Shirai (5), Shinichi Kiyonari (3), Hirokazu Nishida (6), Daisuke Kohda (1), Kosuke Morikawa (7), Yoshizumi Ishino (3)

    1. Medical Institute of Bioregulation, Kyushu University, Fukuoka, Japon 2. PRESTO, JST, Fukuoka, Japon 3. Department of Bioresource and Bioenvironment, Kyushu University, Fukuoka, Japon 4. Research Center for Ultra-High Voltage Electron Microscopy, Osaka University, Osaka, Japon 5. Department of Computer Bioscience, Nagahama Institute of BioScience and Technology, Nagahama, Japon 6. Central Research Laboratory, Hitachi Ltd., Kokubunji, Japon 7. Structural Biology, International Institute for Advanced Studies, Kyoto, Japon

      A number of proteins are involved in DNA replication, which is essential for the inheritance of genetic information. These proteins assemble to form a…
  • The 16th European Microscopy Congress 2016

    Local order and conformation of nucleosomes in solution and in interphase nuclei analysed by cryo-electron microscopy of vitreous sections

    Amélie Leforestier (1), Nicolas Lemercier (1, 2), Françoise Livolant (1)

    1. Laboratoire de Physique des Solides, CNRS, Univ. Paris-Sud, Université Paris-Saclay, Orsay, France 2. IGBMC, CNRS UMR 7104 - Inserm U 964, Illkirch-Graffenstaden, France

    Despite spectacular advances in our knowledge of chromosome dynamics and large scale organisation, little is still known about how the 10 nm nucleosome bead-on-string fibre…
  • The 16th European Microscopy Congress 2016

    Subcellular localization of metal pools determined by TEM-EDS in embryo Arabidopsis thaliana mutants

    Cynthia GILLET (1), Viviane MARY (2), Vanesa SANCHEZ GARCIA DE LA TORRE (2), Sébastien THOMINE (2), Béatrice SATIAT-JEUNEMAÎTRE (2)

    1. Institute for Integrative Biology of the Cell (I2BC)/Imagerie Gif core facility, CNRS, gif sur yvette, France 2. Institute for Integrative Biology of the Cell (I2BC)/Cell Biology Department, CNRS, gif sur yvette, France

    Transmission Electron Microscopy equipped with Energy Dispersive Spectroscopy (TEM-EDS) is known to be a powerful tool to study metal speciation in biological and environmental samples.…
  • The 16th European Microscopy Congress 2016

    Actin-dependent mechanisms during vesicle fusion link exo- and endocytosis in synapses

    Gianvito Arpino (1), Tuomas Näreoja (1), Elena Sopova (1), Oleg Shupliakov (1)

    1. Department of Neuroscience, Karolinska Institutet, Stockholm, Suède

    During the synaptic vesicle fusion at the active zone a fusion pore is formed that results in a formation of an Ω-shape intermediate structure (Ω-profile)…
  • The 16th European Microscopy Congress 2016

    In vitro/ex vivo behavior of a new optical imaging agent targeting αVβ3 integrin.

    Chiara Brioschi (1), Federica Chianale (1), Alessia Cordaro (1), Giovanni Valbusa (2), Federico Maisano (1), Fabio Tedoldi (1)

    1. Bracco Research Center, Bracco Imaging spa, Colleretto Giacosa (TO), Italie 2. Ephoran-Multi Imaging Solutions, colleretto giacosa (TO), Italie

    Introduction Integrin αvβ3 is usually expressed at low or undetectable levels in most adult epithelia, but it is highly upregulated in tumors and correlates with…
  • The 16th European Microscopy Congress 2016

    Flow cytometry, and Transmission electron microscopy; two valuable tools used in an innovative approach to isolate and describe new microorganisms.

    JACQUES BOU KHALIL (1)

    1. INSERM URMITE, APHM, MARSEILLE, France

    Flow cytometry and transmission electron microscopy are two reliable techniques used for decades in microbiology. In parallel, amoebal pathogens like giant viruses, and chlamydiae are…
  • The 16th European Microscopy Congress 2016

    Germ-line cysts organisation in clitellate annelids – from electron microscopy to live cell imaging

    Piotr Świątek (1), Anna Z. Urbisz (1), Karol Małota (1), Szymon Gorgoń (1), Natalia Jarosz (1), Piotr Świątek (1)

    1. Department of Animal Histology and Embryology, University of Silesia, Katowice, Pologne

      At the onset of gametogenesis in animals, the germ-line cells usually form syncytial cysts (clusters). The germ cells in such cysts are interconnected by…
  • The 16th European Microscopy Congress 2016

    Organelle changes in a Huntington’s disease model using cryogenic soft x-ray tomography

    Michele Darrow (1), Wei Dai (2), Patrick Mitchell (3, 4), Imanol Luengo (5), Wah Chiu (4), Elizabeth Duke (1)

    1. Life Science, Diamond Light Source, Harwell, Royaume Uni 2. Department of Cell Biology and Neuroscience, and Center for Integrative Proteomics Research, Rutgers University, New Jersey, Etats-Unis 3. Integrative Molecular and Biomedical Sciences Program, Baylor College of Medicine, Houston, Etats-Unis 4. National Center for Macromolecular Imaging , Baylor College of Medicine, Houston, Etats-Unis 5. The University of Nottingham, Nottingham, Royaume Uni

    Huntington’s Disease, a neurodegenerative disorder characterized by movement and executive function disruption, is linked to an expanded and unstable CAG trinucleotide repeat which translates as…
  • The 16th European Microscopy Congress 2016

    Environmental Scanning Electron Microscopy applied to the identification of asbestos fibers in histological sections

    Caterina Rinaudo (1), Alessandro Croce (1), Nicol Francesca Trincheri (2), Roberta Libener (2), Daniela Degiovanni (3), Federica Grosso (2)

    1. Department of Science and Technological Innovation , University of Eastern Piedmont , Alessandria, Italie 2. Mesothelioma Unit, SS Antonio e Biagio e Cesare Arrigo, General Hospital, Alessandria, Italie 3. Mesothelioma Unit – Supportive and Palliative Care , Country Health Service, Casale Monferrato, Italie

    The strong relation between inhaled asbestos fibers and development of important respiratory diseases (asbestosis, pulmonary carcinoma, mesothelioma) is now demonstrated by many researches and accepted…
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