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Articles tagged "correlative microscopy"

  • The 16th European Microscopy Congress 2016

    Correlative immuno Light Electron Microscopy (CLEM) of subcellular compartments

    Judith Klumperman (1)

    1. Department of Cell Biology, -, Utrecht, Pays-Bas

    Correlative light and electron microscopy (CLEM) methods integrate light and electron microscopy on a single sample, literally bridging the gap between these two microscopy techniques.…
  • The 16th European Microscopy Congress 2016

    An integrated structural cell biology to unravel the vesicle and membrane coat formation mechanism at the inner nuclear envelope.

    Christoph Hagen (1), Tzviya Zeev-Ben-Mordehai (1), Kyle Dent (1, 2), Michael Grange (1), Jens B. Bosse (3, 4), Daven Vasishtan (1), C. Alistair Siebert (1, 5), Rainer Kaufmann (1, 6), Juliana Celeski (1), Cathy Whittle (1), Lothar Schermelleh (6), Barbara Klupp (7), Gerd Schneider (8), Wolfram Antonin (9), Lynn Enquist (10), Thomas C Mettenleiter (7), Kay Grünewald (1)

    1. Wellcome Trust Centre for Human Genetics, Division of Structural Biology, University of Oxford, Oxford, Royaume Uni 2. Life Sciences, Diamond Light Source, Harwell, Royaume Uni 3. Structural Biology of Viruses, Heinrich-Pette-Institiute of Experimental Virology, Hamburg, Allemagne 4. Department of Molecular Biology, Princeton Neuroscience Institute, Princeton University, Princeton, Etats-Unis 5. eBIC, Diamond Light Source, Harwell, Royaume Uni 6. Dept. of Biochemistry, Micron, University of Oxford, Oxford, Royaume Uni 7. Institute for Molecular Virologie and Cell Biology, Friedrich-Loeffler-Institut for Animal Health, Greifswald - Insel Riems, Allemagne 8. BESSY II, Helmhotz-Centre Berlin, Berlin, Allemagne 9. Friedrich Miescher Laboratory, Max Planck Society, Tübingen, Allemagne 10. Department of Molecular Biology, Princeton Neuroscience Institute, Princeton University, Princeton , Etats-Unis

    Our group uses electron cryo microscopy (cryoEM) / tomography (cryoET) and combines these with complementary techniques to understand biological processes mechanistically. The power of such…
  • The 16th European Microscopy Congress 2016

    High-resolution identification of immuno-labelling nanoparticles on tissue using X-ray detection

    Marijke Scotuzzi (1), Jeroen Kuipers (2), Dasha I. Wensveen (1), Pascal de Boer (2), Cornelis W. Hagen (1), Ben N G Giepmans (2), Jacob P. Hoogenboom (1)

    1. Imaging Physics, Delft University of Technology, Delft, Pays-Bas 2. Cell Biology, University Medical Center Groningen, Groningen, Pays-Bas

    Cellular structure can be imaged at nanometer resolution using electron microscopy, but the biological molecules remain invisible in grayscale electron micrographs. With Correlative Light and…
  • The 16th European Microscopy Congress 2016

    Super-resolution fluorescence microscopy of cryo-immobilized samples

    Rainer Kaufmann (1, 2), Christoph Hagen (1), Kay Grünewald (1)

    1. Division of Structural Biology, University of Oxford, Oxford, Royaume Uni 2. Department of Biochemistry, University of Oxford, Oxford, Royaume Uni

    Correlative light and electron microscopy (CLEM) benefits greatly from the development of super-resolution fluorescence microscopy. With a resolution down to the 10 nm range it…
  • The 16th European Microscopy Congress 2016

    Correlative microscopy on animal and vegetal samples with In Resin Fluorescence approach.

    Melina Petrel (1), Clément Chambaud (1), Fabrice Cordelières (1), Jennifer Petersen (1), Etienne Gontier (1), Lysiane Brocard (1)

    1. Bordeaux Imaging Center UMS3420 CNRS, US4 INSERM, Université de Bordeaux, Bordeaux, France

    Understanding the biological role of molecules requires to know their precise localization and structural environment. Thanks to fluorescence microscopy and biotechnologies, the localization of proteins…
  • The 16th European Microscopy Congress 2016

    Novel Linkage Technology of the Shared Alignment Sample Holder for Same Area Observations with Electron Microscopy and Scanning Probe Microscopy

    Ulrich Diestelhorst (1), Takehiro Yamaoka (1), Kazunori Ando (2), Yoichiro Hashimoto (3)

    1. Analytical Application Engineering Section Tokyo 2, Hitachi High-Tech Science Corporation, Kawasaki, Japon 2. Analytical Instruments Engineering Dept., Hitachi High-Tech Science Corporation, Oyama, Japon 3. Application Development Dept., Hitachi High-Technologies Corporation, Hitachinaka, Japon

        We developed an innovative air protection sample holder enabling a hermeneutically sealed sample transfer from Hitachi’s ion milling instrument to the Field Emission Scanning…
  • The 16th European Microscopy Congress 2016

    An absolute sample position referencing solution for convenient cross-platform observations; application to the assessment of microscope stability and translation stage reproducibility

    Olivier Acher (1), Alexander Podzorov (1), Alexandre Grigoriev (1)

    1. HORIBA Jobin Yvon, Palaiseau, France

    We present the prototype of an instrument add-on that tracks the absolute position and orientation of a sample, and that can be implemented on several…
  • The 16th European Microscopy Congress 2016

    A Correlative Methodology based on SIMS for Advanced Materials Characterization

    Santhana Eswara (1), Rong Hu (2), Lluís Yedra (1), Jean-Nicolas Audinot (1), Alexander Schwedt (3), Cem Tasan (4), Joachim Mayer (3), Dierk Raabe (2), Tom Wirtz (1)

    1. Materials Research and Technology, Luxembourg Institute of Science and Technology, Belvaux, Luxembourg 2. Max-Planck-Institut für Eisenforschung GmbH, Düsseldorf, Allemagne 3. Central Facility for Electron Microscopy, RWTH Aachen University, Aachen, Allemagne 4. Dept. of Materials Science and Engineering, Massachusetts Institute of Technology, Cambridge, MA, Etats-Unis

    Development and innovations of modern high-performance materials rely crucially on the repertoire of characterization methods available. While individual techniques provide information on a specific aspect…
  • The 16th European Microscopy Congress 2016

    High Resolution Chemical Imaging on the Helium Ion Microscope

    David Dowsett (1), Jean-Nicolas Audinot (1), Florian Vollnhals (1), Santhana Eswara (1), Tom Wirtz (1)

    1. Materials Research and Technology, Luxembourg Institute of Science and Technology, Belvaux, Luxembourg

    The ORION NanoFab helium ion microscope (HIM) has become an ideal tool for imaging and nano-patterning tool owing to its high lateral resolution [1]. Helium…
  • The 16th European Microscopy Congress 2016

    Tracking tumor metastasis in vivo at high-resolution

    Jacky GOETZ (1)

    1. INSERM U1109, None, STRASBOURG, France

    Three reasons explain why most of the critical events driving normal and pathological scenarios had been less investigated: they occur rarely in space and time,…
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