Articles tagged "correlative light and electron microscopy"

The 16th European Microscopy Congress 2016
3-D EM exploration of the hepatic microarchitecture – lessons learned for large-volume in situ serial sectioning
Gerald Shami (1), Delfine Cheng (1), Minh Huynh (2), Celien Vreuls (3), Eddie Wisse (2, 4, 5), Filip Braet (1, 2, 6)
1. School of Medical Sciences (Discipline of Anatomy and Histology) – The Bosch Institute, The University of Sydney, Sydney, Australie 2. Australian Centre for Microscopy and Microanalysis, The University of Sydney, Sydney, Australie 3. Department of Pathology, Amphia Hospital, Breda, Maastricht, Pays-Bas 4. Maastricht Multimodal Molecular Imaging Institute, Division of Nanoscopy, University of Maastricht, Maastricht, Pays-Bas 5. Department of Internal Medicine, University of Maastricht, Maastricht, Pays-Bas 6. Cellular Imaging Facility, Charles Perkins Centre, The University of Sydney, Sydney, Australie
To-date serial block-face scanning electron microscopy (SBF-SEM) dominates as the premier technique for generating three-dimensional (3-D) data of resin-embedded biological samples at an unprecedented resolution…
The 16th European Microscopy Congress 2016
Mycobacterial protein weaponry studied by cryo-correlative microscopy
Kristof Notelaers (1), Casper Berger (1), Nino Iakobachvili (1), Delei Chen (1), Axel Siroy (1), Hirotoshi Furusho (1), Raimond Ravelli (1), Carmen López-Iglesias (1), Peter Peters (1)
1. M4I Division of Nanoscopy, Maastricht University, Maastricht, Pays-Bas
In correlative light and electron microscopy, the discerning power of fluorescence microscopy (FM) is combined with the ultra-high resolution of electron microscopy (EM). We fluorescently…
The 16th European Microscopy Congress 2016
Electron-beam induced fluorescence superresolution with 100nm resolution in CLEM on labelled tissue sections
Lennard M. Voortman (1, 2), Aditi Srinivasa Raja (1), Aaro Väkeväinen (1), Pascal de Boer (3), Ben N.G. Giepmans (3), Pieter Kruit (1), Jacob P. Hoogenboom (1)
1. Faculty of Applied Sciences , Delft University of Technology, Delft, Pays-Bas 2. DELMIC B.V., Delft, Pays-Bas 3. Department of Cell Biology, University Medical Center Groningen, University of Groningen , Groningen, Pays-Bas
We present a novel optical superresolution (SR) technique using integrated correlative light and electron microscopy. Recent advances in SR techniques has revolutionized the field of…
The 16th European Microscopy Congress 2016
3D mapping of subcellular structures with super-resolution array tomography
Sebastian Markert (1), Sebastian Britz (1), Sven Proppert (2, 3), Marietta Lang (1), Daniel Witvliet (4, 5), Ben Mulcahy (4, 5), Markus Sauer (2), Mei Zhen (4, 5), Jean-Louis Bessereau (6), Christian Stigloher (1)
1. Biocenter, Division of Electron Microscopy, University of Würzburg, Würzburg, Allemagne 2. Department of Biotechnology and Biophysics, University of Würzburg, Würzburg, Allemagne 3. Department of Neurophysiology, Institute of Physiology, University of Würzburg, Würzburg, Allemagne 4. Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital, Toronto, Canada 5. Department of Molecular Genetics, Physiology and Institute of Medical Science, University of Toronto, Toronto, Canada 6. Institut NeuroMyoGene, Université Claude Bernard Lyon 1, Villeurbanne, France
The combination of fluorescence light microscopy (FLM) and electron microscopy (EM) makes it possible to put molecular identity in its full ultrastructural context. With array…
The 16th European Microscopy Congress 2016
Integrated microscopy: Matching scales and capabilities in light and electron microscopy.
Jacob Hoogenboom (1)
1. Imaging Physics, Delft University of Technology, Delft, Pays-Bas
Superresolution techniques have pushed the resolution of fluorescence microscopy (FM) towards that of electron microscopy (EM)[1]. Meanwhile, developments in scanning EM (SEM) are revolutionizing EM,…
The 16th European Microscopy Congress 2016
Power of FIB-SEM Tomography for Biological Samples
Caroline Kizilyaprak (1), Florence Niedergang (2), Damien De Bellis (1), Willy Blanchard (1), Jean Daraspe (1), Niko Geldner (3), Bruno Humbel (1)
1. Electron Microscopy Facility, University of Lausanne, Lausanne, Suisse 2. Inserm U1016-CNRS UMR8104- Université Paris Descartes, Institut Cochin, Paris, France 3. Plant Cell Biology Lab - DBMV, University of Lausanne, Lausanne, Suisse
Three-dimensional (3-D) spatial distribution of organelles within cells at nanometer resolution is essential to better understand cellular processes and functions. Currently, FIB-SEM tomography provides a…