Articles tagged "CLEM"

The 16th European Microscopy Congress 2016
Multi-color correlative PALM/STORM and electron tomography reveals micro-domain organization of endosomes
Nicolas Brouilly (1), Yannis Kalaidzidis (1), Jean-Marc Verbavatz (2), Marino Zerial (1)
1. MPI-CBG, Zerial lab, Dresden, Allemagne 2. MPI-CBG, Electron Microscopy Facility, Dresden, Allemagne
Correlative Light and Electron Microscopy (CLEM) is a method of choice to demonstrate the localization of a protein in a given organelle while revealing the…
The 16th European Microscopy Congress 2016
Super-resolution fluorescence microscopy of cryo-immobilized samples
Rainer Kaufmann (1, 2), Christoph Hagen (1), Kay Grünewald (1)
1. Division of Structural Biology, University of Oxford, Oxford, Royaume Uni 2. Department of Biochemistry, University of Oxford, Oxford, Royaume Uni
Correlative light and electron microscopy (CLEM) benefits greatly from the development of super-resolution fluorescence microscopy. With a resolution down to the 10 nm range it…
The 16th European Microscopy Congress 2016
Correlative microscopy on tissue: Development applied to the skin and the nervous system.
Etienne GONTIER (1), Jimmy LEDIGABEL (2), Sabrina LACOMME (1), Marie GLAVIER (1), Gwendal JOSSE (2), Marc LANDRY (1, 3)
1. Bordeaux Imaging Center - Pôle d'imagerie électronique - CNRS UMS 3420 -, Université de Bordeaux - CNRS , Bordeaux, France 2. Centre de recherche sur la peau - service bio-physique et imagerie, Pierre Fabre Dermo-Cosmétique, Toulouse, France 3. Institute for Interdisciplinary Neuroscience - CNRS UMR 5297, CNRS - Université de Bordeaux, Bordeaux, France
Correlative Light and Electron Microscopy (CLEM) combines two imaging techniques at different scales to figure out the precise localization of rare elements in a well-defined…
The 16th European Microscopy Congress 2016
Improving 3d correlation in integrated correlated light and electron microscopy using confocal laser scanning microscopy
Josey Sueters-Di Meo (1), Pieter Kruit (1), Jacob Hoogenboom (1)
1. ImPhys - Charged Particle Optics, Delft University of Technology, Delft, Pays-Bas
In CLEM experiments biological samples are typically first investigated by light microscopy (LM) to locate a region of interest. Next, nanometer-scale imaging can be achieved…
The 16th European Microscopy Congress 2016
Cathodoluminescence microscopy of biological samples for correlative light and electron microscopy (CLEM) using organic fluorophores
Christopher Schmid (1, 2), Klaus Yserentant (1, 3, 4), Lucian Stefan (5), Dirk-Peter Herten (3, 4), Rasmus Schröder (1, 2)
1. Centre for Advanced Materials, University of Heidelberg, Heidelberg, Allemagne 2. CellNetworks, CryoEM, Heidelberg University Hospital, Heidelberg, Allemagne 3. CellNetworks, Single Molecule Spectroscopy, Heidelberg University Hospital, Heidelberg, Allemagne 4. Department of Physical Chemistry, University of Heidelberg, Heidelberg, Allemagne 5. Corporate Research and Technology, Carl Zeiss AG, Oberkochen, Allemagne
A CLEM study in biology aims at providing knowledge on the identity and localisation of cellular constituents of interest within a cell's ultrastructure. The key…
The 16th European Microscopy Congress 2016
Nanotomy and CLEM techniques shed new light on biomedical processes
Ben Giepmans (1)
1. Dept. Cell Biology, UMC Groningen, Groningen, Pays-Bas
A spectrum of dyes and probes now enable to localize molecules of interest within living cells by fluorescence microscopy. With electron microscopy (EM), cellular ultrastructure…