Macrobrachium rosenbergii Nodavirus (MrNV) causes white tail disease (WTD) in the giant freshwater prawn M. rosenbergii. In our past studies, we generated Macrobrachium rosenbergii Nodavirus virus-like particles (MrNV-VLPs) that were capable of being “nanocontainers” through the uptake of foreign plasmids after viral capsid disassembling/reassembling treatment. Nevertheless, the studies into the binding and internalization of this particular virus into susceptible host cells have been lacking; therefore our team has utilized live MrNV and recombinantly synthesized MrNV-VLPs as a tool to examine viral entry mechanisms in Sf9 insect cells; whereby revealing the dependence upon the caveolin-mediated endocytotic pathway for internalization and infection. Furthermore, viral capsid protein subunit modeling and capsid enzyme digestion were performed for revealing viral protrusions/ligands.
To cite this abstract:MONSICHA SOMRIT, ATTHABOON WATTHAMMAWUT, WATTANA WEERACHATYANUKUL, YANN GUERARDEL; Study of the interaction of Macrobrachium rosenbergii Nodavirus and its virus-like particles (MrNV-VLPs) in Sf9 insect cells: the dependence upon caveolin-mediated endocytosis and the possible existence of a capsid ligand domain. The 16th European Microscopy Congress, Lyon, France. https://emc-proceedings.com/abstract/study-of-the-interaction-of-macrobrachium-rosenbergii-nodavirus-and-its-virus-like-particles-mrnv-vlps-in-sf9-insect-cells-the-dependence-upon-caveolin-mediated-endocytosis-and-the-possible-existen/. Accessed: September 21, 2023
EMC Abstracts - https://emc-proceedings.com/abstract/study-of-the-interaction-of-macrobrachium-rosenbergii-nodavirus-and-its-virus-like-particles-mrnv-vlps-in-sf9-insect-cells-the-dependence-upon-caveolin-mediated-endocytosis-and-the-possible-existen/