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Probing the multiscale structure, composition and nanomechanical properties of lipids and biopolymers in natural systems: a few examples.

Abstract number: 6983

Session Code: IM10-OP176

DOI: 10.1002/9783527808465.EMC2016.6983

Meeting: The 16th European Microscopy Congress 2016

Session: Instrumentation and Methods

Topic: Correlative microscopy

Presentation Form: Oral Presentation

Corresponding Email: cedric.gaillard@nantes.inra.fr

Cédric Gaillard (1)

1. BIA Microscopy Facilities, INRA, Nantes, France

Keywords: AFM, direct and indirect correlation, Raman microspectrometry, sample preparation, SEM/EDX, STXM, TEM

The natural systems as plants or animal tissues possess a highly complex organisation of numerous components: micronutriments, proteins, polyssacharides, lipids, water, ….  Their natural arrangement gives very fine structures that range from the nanometer up to few meters scale range (typically from the protein to the whole plant). Native architectures have a direct influence on the functional, chemical, organoleptic and nutritional macroscopic properties relevant for designing sustainable performant products for environmentally compatible food and non–food uses.

The development and application of experimental methodologies to probe the assemblies of lipids, biopolymers, mineral nutrients as well as structural water from the sub-cellular to the molecular levels is then a permanent challenge. The parallel use of a set of complementary microscopy tools that provides relevant information may be needed to overcome the complexity of hydrated natural systems and to get both their structural, composition and nanomechanical properties. Most of the time, the experimental sample preparation is also a critical prerequisite to adapt the biological samples to the features of the analytical tool while keeping as most as possible the structures in their native shape. Thanks to this step, the microscopy tools can be operated through a direct or indirect correlation approach.

Using few applications we developed on biopolymer-based nanoparticles as well as animal cells or plant tissues (see Figure 1), we will show how to probe the structure/composition/nanomechanical properties focusing on the strong complementarity of AFM imaging and force mapping, Raman mapping, SEM/EDX, and scanning transmission X-ray spectrometry (STXM). We will also discuss about the samples preparation strategies, and about data processing in relation to the information expected.

 

 

References

M. Gayral, C. Gaillard, B. Bakan, M. Dalgalarrondo, K. Elmorjani, C. Delluc, S. Brunet, L. Linossier, M.H. Morel, D. Marion, Transition from vitreous to floury endosperm in maize (Zea mays L.) kernels is related to protein and starch gradients, Journal of Cereal Science, 2016, accepté

G. Philippe, C. Gaillard, J. Petit, N. Geneix, M. Dalgalarrondo, R. Franke, C. Rothan, L. Schreiber, D. Marion, B. Bakan, Ester-crosslink Profiling of the Cutin Polymer of Wild Type and Cutin Synthase Tomato (Solanum lycopersicum L.) Mutants Highlights Different Mechanisms of Polymerization, Plant Physiology

C. Karunakaran, C.R. Christensen, C. Gaillard, R. Lahlali, L.M. Blair, V. Perumal, S.S Miller, A.P. HitchcockIntroduction of soft X-ray spectromicroscopy as an advanced technique for plant biopolymers research. PLoS One, 2015 26;10 (3) pages: e0122959.

Covis R., Vives T., Gaillard C., Maud Benoit, Benvegnu T., Interactions and hybrid complex formation of anionic algal polysaccharides with a green cationic glycine betaine derived surfactant, Accepted in Carbohydrate Polymers, 2015, May 5;121:436-48.

Figures:

Fig. 1. AFM/Raman/Fluorescence correlative microscopy (left image) of the native wheat endosperm tissue, and detail of the Raman chemical map (top right image) and error-signal AFM image (bottom right image).

To cite this abstract:

Cédric Gaillard; Probing the multiscale structure, composition and nanomechanical properties of lipids and biopolymers in natural systems: a few examples.. The 16th European Microscopy Congress, Lyon, France. https://emc-proceedings.com/abstract/probing-the-multiscale-structure-composition-and-nanomechanical-properties-of-lipids-and-biopolymers-in-natural-systems-a-few-examples/. Accessed: September 21, 2023
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