EMC Abstracts

Official abstracts site for the European Microscopy Congress

MENU 
  • Home
  • Meetings Archive
    • The 16th European Microscopy Congress 2016
  • Keyword Index
  • Your Favorites
    • Favorites
    • Login
    • Register
    • View and Print All Favorites
    • Clear all your favorites
  • Advanced Search

Multiscale investigation of USPIO nanoparticles in atherosclerotic plaques and their catabolism and storage in vivo

Abstract number: 6669

Session Code: MS00-491

DOI: 10.1002/9783527808465.EMC2016.6669

Meeting: The 16th European Microscopy Congress 2016

Session: Materials Science

Topic: Nanoparticles: from synthesis to applications

Presentation Form: Poster

Corresponding Email: maraloiu@infim.ro

Valentin-Adrian Maraloiu (1), Florence Appaix (2), Alexis Broisat (3), Dominique Le Guellec (4), Valentin Serban Teodorescu (1), Catherine Ghezzi (3), Boudewijn van der Sanden (5), Marie Genevieve Blanchin (6)

1. Laboratory of Atomic Structures and Defects in Advanced Materials, National Institute of Materials Physics, Magurele, Roumanie 2. Two-Photon Microscopy Platform, IBiSA-ISdV, Grenoble Institute of Neuroscience INSERM U836, Grenoble, France 3. Radiopharmaceutiques Biocliniques Lab., Faculté de Médecine, La Tronche, France 4. Institut Biologie et Chimie des Protéines, Lyon, France 5. Clinatec, Grenoble, France 6. Institut Lumière Matière, Lyon, France

Keywords: Atherotic plaque, MRI contrast agent, TEM, Two-Photon Laser Scanning Microscopy and Nanotechnology, USPIO nanoparticles

Since applications of nanotechnologies for life and health sciences are booming, magnetic nanoparticles (NP) have undergone considerable development. They combine physical, chemical and magnetic properties that make them appropriate as contrast agents  for diagnosis using medical imaging techniques such as Magnetic Resonance Imaging (MRI) or ultrasonic imaging. Nanometric contrast agents are made of metallic rare earth elements (Gd) or transition metal (Mn, Fe) oxide cores.

      The storage and catabolism of Ultrasmall Super Paramagnetic Iron Oxide (USPIO) nanoparticles was analyzed through a multiscale approach combining Two Photon Laser Scanning Microscopy (TPLSM) and High-Resolution Transmission Electron Microscopy (HRTEM) at different times after intravenous injection (iv) in an atherosclerotic ApoE-/- mouse model. The atherotic plaque features and the USPIOs heterogeneous biodistribution were revealed from organ’s scale down to subcellular level. The biotransformation of the nanoparticle iron oxide (maghemite) core into ferritin, the non-toxic form of iron storage, was demonstrated for the first time ex vivo in atherosclerotic plaques as well as in spleen, the iron storage organ. These results rely on an innovative spatial and structural investigation of USPIO’s catabolism in cellular phagolysosomes. This study showed that these nanoparticles were stored as non-toxic iron compounds: maghemite oxide or ferritin, which is promising for MRI detection of atherosclerotic plaques in clinic using these USPIOs [1].

 

 

References

 

[1] VA Maraloiu, F Appaix, A Broisat et al., Nanomedicine: Nanotechnology, Biology and Medicine 12 (1), 191-200 (2016)

 

 

Acknowledgement

 

 The two-photon microscopy studies were performed on the National Platform of Intravital Microscopy in Grenoble (France Life Imaging). This platform was partly funded by the French program “Investissement d’Avenir” run by the “Agence Nationale pour la Recherche”; grant “Infrastructure d’avenir en Biologie Santé – ANR11-INBS-0006”. Researches were partially granted by the French National Agency for Research (ANR) in the frame of the INFLAM project and partially supported by a grant of the Romanian National Authority for Scientific Research, CNCS – UEFISCDI, project number PN-II-RU-PD-2011-3-0067.

Figures:

Figure 1. a) Z-projections (standard deviation fluorescence intensities) of 68 TPLSM images with a slice spacing of 3 μm of atherosclerotic plaques (class 4: atheroma) in non-fixed aortic rings 20 days after USPIOs iv injection. b) Z-projection (average fluorescence intensities) of 47 TPLSM images with a slice spacing of 3 μm of an atherosclerotic plaque (P) in a PFA-fixed aortic ring (W: aortic wall). c) Zoom of white rectangle region in a), showing that USPIO particles were mostly detected under collagen cap (blue, endogenous SHG signals). d) Zoom of white rectangle region in b), showing distribution of USPIO particles (circles) in atherosclerotic plaque.

Figure 2. a) CTEM overview in atherosclerotic plaque at D20 revealing several vesicles; a central vesicle (white arrow) enlarged in (b) having internalised USPIOs, electron diffraction pattern (maghemite)(c); (d) Fitting of size distribution (percentage of particle number Np with a given size over total number of counted particles Nt) for non transformed USPIOs in plaque from CTEM images.

Figure 3. High magnification HRTEM image from the vesicle in the atherosclerotic plaque at D20 reveals details of crystallized USPIOs, with FFTs confirming the maghemite structure and revealing the orientation of the particles A and B.

To cite this abstract:

Valentin-Adrian Maraloiu, Florence Appaix, Alexis Broisat, Dominique Le Guellec, Valentin Serban Teodorescu, Catherine Ghezzi, Boudewijn van der Sanden, Marie Genevieve Blanchin; Multiscale investigation of USPIO nanoparticles in atherosclerotic plaques and their catabolism and storage in vivo. The 16th European Microscopy Congress, Lyon, France. https://emc-proceedings.com/abstract/multiscale-investigation-of-uspio-nanoparticles-in-atherosclerotic-plaques-and-their-catabolism-and-storage-in-vivo/. Accessed: May 17, 2022
  • Tweet
  • Email
  • Print
Save to PDF

« Back to The 16th European Microscopy Congress 2016

EMC Abstracts - https://emc-proceedings.com/abstract/multiscale-investigation-of-uspio-nanoparticles-in-atherosclerotic-plaques-and-their-catabolism-and-storage-in-vivo/

Most Viewed Abstracts

  • mScarlet, a novel high quantum yield (71%) monomeric red fluorescent protein with enhanced properties for FRET- and super resolution microscopy
  • 3D structure and chemical composition reconstructed simultaneously from HAADF-STEM images and EDS-STEM maps
  • Pixelated STEM detectors: opportunities and challenges
  • Layer specific optical band gap measurement at nanoscale in MoS2 and ReS2 van der Waals compounds by high resolution electron energy loss spectroscopy
  • Developments in unconventional dark field TEM for characterising nanocatalyst systems

Your Favorites

You can save and print a list of your favorite abstracts by clicking the “Favorite” button at the bottom of any abstract. View your favorites »

Visit Our Partner Sites

The 16th European Microscopy Congress

The official web site of the 16th European Microscopy Congress.

European Microscopy Society

European Microscopy Society logoThe European Microscopy Society (EMS) is committed to promoting the use and the quality of advanced microscopy in all its aspects in Europe.

International Federation of Societies for Microscopy

International Federation of Societies for Microscopy logoThe IFSM aims to contribute to the advancement of microscopy in all its aspects.

Société Française des Microscopies

Société Française des MicroscopiesThe Sfµ is a multidisciplinary society which aims to improve and spread the knowledge about Microscopy.

Connect with us

Imaging & Microscopy
Official Media Partner of the European Microscopy Society.

  • Help & Support
  • About Us
  • Cookies & Privacy
  • Wiley Job Network
  • Terms & Conditions
  • Advertisers & Agents
Copyright © 2022 John Wiley & Sons, Inc. All Rights Reserved.
Wiley
loading Cancel
Post was not sent - check your email addresses!
Email check failed, please try again
Sorry, your blog cannot share posts by email.