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Modified University of Wisconsin Solution With Melatonin and the Its Efficacy of Kidney Preservation Time

Abstract number: 6441

Session Code: LS08-097

DOI: 10.1002/9783527808465.EMC2016.6441

Meeting: The 16th European Microscopy Congress 2016

Session: Life Sciences

Topic: Human health and disease

Presentation Form: Poster

Corresponding Email: arbaks@yahoo.com

Abdurrahman Coskun (1), Cumhur Yegen (2), Serap Arbak (3), Omer Gunal (2), Wafi Attaallah (2), Ismail Seckin (4), Banu Cetiner (1), Zeynep Yildirim (1), Yigit Erdemgil (1), Merve Acikel Elmas (3), Sibel Demirci (5), Ozge Can (6), Mustafa Serteser (1), Aysel Ozpınar (1), Gulcin Basdemir (7), Ahmet Belce (8), Ibrahim Unsal (1)

1. School of Medicine, Department of Medical Biochemistry, Acibadem University, Istanbul, Turquie 2. School of Medicine, Department of General Surgery, Marmara University, Istanbul, Turquie 3. School of Medicine, Department of Histology and Embryology, Acibadem University, Istanbul, Turquie 4. Cerrahpasa Faculty of Medicine, Department of Histology and Embryology, Istanbul University, Istanbul, Turquie 5. Cerrahpasa Faculty of Medicine, Department of Histology and Embryology, Istanbul University, Istanbul, Turquie 6. Faculty of Engineering, Department of Medical Engineering, Acibadem University, Istanbul, Turquie 7. Department of Pathology, Memorial Hospital, Istanbul, Turquie 8. Faculty of Health Sciences, Bezmialem Vakıf University, Istanbul, Turquie

Keywords: kidney, Lactate Dehydrogenase, microscopy, University of Wisconsin Solution

Organ transplantation solutions are used for protection of organ functions for a long time. In order to provide long lasting time period for organ preservation, donor organs are kept in special preservation solutions such as University of Wisconsin (UW), Histidine-Triptophane-Ketogluterate (HTK) and Celcior solutions. Although UW is the most preferred solution, preservation time even for the kidney tissue, has not exceeded over 36 hours. The aim of this study is to extend the preservation time and also provide more effective protection. In order to provide better preservation,  the ingredients of the UW solution were changed (Mod UW), as well as melatonin was included (Mod UW+M)  in the preservation medium. Time-related morphological changes of rat kidneys in UW (group I), Modified UW (group II) and Modified UW+Melatonin (Mod UW+M) (group III) were comparatively investigated within this study. Totally perfused kidneys were removed and placed in UW, Mod UW and Mod UW +M solutions and kept during 2, 10, 24 and 72 h at 4⁰ C. Kidney tissue samples were taken at all given time intervals and these samples were prepared for paraffin sections. Kidney tissue samples, taken at 72nd hour, were fixed in 2.5% glutaraldehyde and prepared for epoxy resin embedding. Histopathological scoring system were performed for light microscopical evaluation by using a scale ranging from 0 to 3 (0: none; 1: mild; 2: moderate; and 3:severe) for each criterion. Histopathological parameters were renal injury, based on tubular and glomerular degeneration, inflammatory cell infiltration and vasocongestion. Liquid samples, taken at 2, 10, 24 and 72 h at 4⁰ C from the storage media, investigated for lactate dehydrogenase (LDH) activity which was measured using standard chemistry method (Siemens ADVIA 1800 chemistry System). Comparative findings at light and transmission electron microscopical levels revealed that preservation in Mod UW+M solution was statistically much more prominent in all time intervals, significantly at 72nd hour of preservation.  In all groups, LDH levels were consistent with morphological results. We could conclude that Mod UW+M was the most effective solution among the  experimental groups, especially  suitable for preservation up to 72 h. In this study,  the important efficacies of UW solution, a better protectant and melatonin,  an effective agent to be used in such systems, were highlighted.

References

1. Goktas C, Coskun A, Bicik Z, Horuz R, Unsal I, Serteser M, Albayrak S, Sarica K. Evaluating ESWL-induced renal injury based on urinary TNF-α, IL-1α, and IL-6 levels. Urol Res, 40: 569–73, 2012

2. Gunal O, Coskun A, Aslaner A, Yildirim U. Does melatonin alleviate cold preservation injury of the liver Turk J Med Sci, 40: 465–70, 2010

3. S Öner, F Ercan, S Arbak: time-dependent morphological alterations of cold-stored small bowel in Euro-Collins and Ringer’s lactate solutions. Acta Histochemica, 10: 207–217,2004

This study was financed by The Scientific and Technological Research Council of Turkey (TUBITAK), project number 113S847

To cite this abstract:

Abdurrahman Coskun, Cumhur Yegen, Serap Arbak, Omer Gunal, Wafi Attaallah, Ismail Seckin, Banu Cetiner, Zeynep Yildirim, Yigit Erdemgil, Merve Acikel Elmas, Sibel Demirci , Ozge Can, Mustafa Serteser, Aysel Ozpınar, Gulcin Basdemir, Ahmet Belce, Ibrahim Unsal; Modified University of Wisconsin Solution With Melatonin and the Its Efficacy of Kidney Preservation Time. The 16th European Microscopy Congress, Lyon, France. https://emc-proceedings.com/abstract/modified-university-of-wisconsin-solution-with-melatonin-and-the-its-efficacy-of-kidney-preservation-time/. Accessed: January 25, 2021
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