Recently near-field techniques play a fundamental role in Nanoscience microscopy. Two different ways exist to make infrared studies with near-field techniques: optical techniques measuring the transmitted signal coming from the nano-object or photothermal approachs using thermometer to link temperature to absorption measurements. Considering the limitations of the optical techniques, we have developed in our team an innovative photothermal technique called AFM-IR1. The AFM-IR technique is a user-friendly benchtop technique that enables infrared spectroscopy with a spatial resolution well below conventional optical diffraction limits. It acquires IR absorption imaging spectrally resolved with lateral resolution of tens of nanometer2.
First we will present the experimental set-up and some of the critical technical sides and then illustrate technological advances. Then we will show the result obtained on biodegradable polymeric nanoparticles. This work has been done in collaboration with the Institut of molecular science (ISMO). We have used this technique to characterize the composition of the nanoparticles (drug encapsulation, presence of lignad) and to localize them inside fixed macrophages at the subcellular scale.
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To cite this abstract:Jérémie Mathurin, Elisabetta Pancani, Ariane Deniset-Besseau, Ruxandra Gref, Alexandre Dazzi; IR Nano-spectro-imaging: Characterization of polymeric nanoparticles and in vitro study of their interaction with cells.. The 16th European Microscopy Congress, Lyon, France. https://emc-proceedings.com/abstract/ir-nano-spectro-imaging-characterization-of-polymeric-nanoparticles-and-in-vitro-study-of-their-interaction-with-cells/. Accessed: September 20, 2021
EMC Abstracts - https://emc-proceedings.com/abstract/ir-nano-spectro-imaging-characterization-of-polymeric-nanoparticles-and-in-vitro-study-of-their-interaction-with-cells/