Background. The existence of nanobacteria as a separate systematic unit is still under the doubt (1, 2). Nanobacteria are characterized as mycoplasmas and as L-transformed forms, too (3).
The aim of current investigations was to evaluate the possibilities of formation of “nanostructure – nanobacteria” in overgrowth bacterial cultures (death phase of growth) and in the presence of antibiotics.
Material and methods. E. coli1257 and S. flexnerii130 were used in these investigations to follow the changes in bacterial membrane structures in death phase of growth and after the treatment of antibiotics. Bacteria were grown anaerobically in LB medium (48 hours, 37Co), solidified with 1.8% agar when necessary. The following antibiotics were used: tetracycline (15 µg/ml), doxycycline (15 µg/ml), amoxicillin (25 µg/ml), ampicillin (35 µg/ml), cefoxitin (50 µg/ml), kanamycin (50 µg/ml), gentamicin (50 µg/ml), chloramphenicol (30 µg/ml), and streptomycin (50µg/ml).
Transmission (TEM) electron microscopy technique was used to evaluate the changes in bacterial membrane structures.
Results. The formation of spherical granules in 40-100nm surrounding by membrane structures of S. flexnerii in growth death phase (Fig. 1) and formation of outgrowths on E. coli cell walls with different length during the ampicillin-treatment (Fig. 2) were detected in these studies. These formations could be discussed as a basis for the development of nanobacteria under the specific conditions of environment.
. Robert J.C, Mc Lean, Brenda L. Kirland (2014) Nanostructure and Nanobacteria.p.1-10, Nanomicrobiology: Physological and Environmental CharacteristicsL\ Eds. Lary Barton, Demis Bazilski, Huifang Xu. 125p. DOI 10.1007/978-14939-16672-1
. Jan Martel; Hsin-Hsin Peng; David Young; Cheng-Yeu Wu; John D Young. Of nanobacteria, nanoparticles, biofilms and their role in health and disease: facts, fancy and future.Nanomedicine. 2014;9(4):483-499.
. VD Timakov, G.YA Kagan (1973) L-form bacteria and bacteria family Mycoplas-mataceae pathology. Ed. “Medicine”. Moscow, 385C.
To cite this abstract:Margarita Hovnanyan, Astghik Pepoyan, Karlen Hovnanyan; Formation of bacterial nanostructures: the impact of growth dead phase and antibiotics. The 16th European Microscopy Congress, Lyon, France. https://emc-proceedings.com/abstract/formation-of-bacterial-nanostructures-the-impact-of-growth-dead-phase-and-antibiotics/. Accessed: September 20, 2021
EMC Abstracts - https://emc-proceedings.com/abstract/formation-of-bacterial-nanostructures-the-impact-of-growth-dead-phase-and-antibiotics/