Membrane proteins play crucial roles in many cellular processes such as signaling, nutrient uptake and cell adhesion. Although the lipid bilayer influences many aspects of membrane protein function, our understanding of lipid–protein interactions is limited. In the first part of my talk, I will describe how electron crystallography of the water channel aquaporin-0 reconstituted with lipids into two-dimensional crystals can be used to address very basic questions in membrane biology, such as the driving forces that define lipid–protein interactions and the effects of hydrophobic mismatch. In the second part, I will discuss how we use membrane proteins reconstituted into nanodiscs to make it possible to study lipid–protein interactions by single-particle cryo-electron microscopy. In particular, I will discuss how the mechanosensitive channel MscS adapts to different lipid bilayers.
To cite this abstract:Thomas Walz; Electron microscopy approaches to studying lipid–protein interactions. The 16th European Microscopy Congress, Lyon, France. https://emc-proceedings.com/abstract/electron-microscopy-approaches-to-studying-lipid-protein-interactions/. Accessed: December 6, 2022
EMC Abstracts - https://emc-proceedings.com/abstract/electron-microscopy-approaches-to-studying-lipid-protein-interactions/