Dynamic-Transmission Electron Microscopy at the Relativistic Electron Gun for Atomic Exploration (REGAE) for live cell imaging

Abstract number: 6933

Session Code: IM03-OP101

DOI: 10.1002/9783527808465.EMC2016.6933

Meeting: The 16th European Microscopy Congress 2016

Session: Instrumentation and Methods

Presentation Form: Oral Presentation

Corresponding Email: stephanie.manz@mpsd.mpg.de

Stephanie Manz (1), Sercan Keskin (1), Stephanie Besztejan (2), Benno Zeitler (3), Sana Azim (1), Guenther Kassier (1), Robert Buecker (1), Deybith Venegas-Rojas (4), Svenja Riekeberg (4), Dongfang Zhang (1), Albert Casandruc (1), Rolf A. Loch (1), Yinpeng Zhong (1), Hossein Delsim-Hashemi (5), Sascha W. Epp (1), Klaus Floettmann (5), Hoc Khiem Trieu (4), Andrea Rentmeister (6), R. J. Dwayne Miller (1)

1. MPI for the Structure and Dynamics of Matter, CFEL, Hamburg, Allemagne 2. University of Hamburg, Chemistry Department, Hamburg, Allemagne 3. University of Hamburg, Institut für Experimentalphysik , Hamburg, Allemagne 4. Hamburg University of Technology , Institute of Microsystems Technology, Hamburg, Allemagne 5. MPY, DESY, Hamburg, Allemagne 6. Westfaelische Wilhelms-Universitaet Muenster, Institute of Biochemistry, Muenster, Allemagne

Keywords: DTEM, environmental TEM, HVEM

With the relativistic electron gun for atomic exploration (REGAE) we seek to observe structural dynamics both in real space imaging and diffraction [1]. REGAE is based on a RF gun accelerator and operates in the range from 2 to 5 MeV. RF gun technology allows high brightness for electron pulses at high energy. The machine is equipped with an RF cavity system, allowing for picosecond bunches with energy spread compensation to prevent limitations by chromatic aberrations and space charge. A custom made lens system for 3 MeV pulse energy system is set in place.

The presentation will include a discussion on space charge induced aberrations in dynamic HVEM, give estimates about the anticipated resolution and discuss the prospects for dynamic transmission electron microscopy of organic samples in environmental cells. Recent results making use of nano-fluidic cell technology developed in house will be presented as an outlook: DNA – nanoparticle multimers have been studied regarding their dynamic in liquid and stability under electron irradiation during TEM imaging in a conventional 200 keV microscope [2]. First experiments towards imaging dynamics in living cancer cells under environmental conditions in the TEM will be discussed [3].

References

[1] S. Manz et al. Faraday Discuss. 2015, 177, 467-491.

[2] S. Keskin et al., J. Phys. Chem. Lett. 2015, 6, 4487−4492.

[3] S. Besztejan et al., submitted, 2016.

Figures:

Estimate of resolution anticipated with REGAE TEM depending on electron bunch length st and focal length of objective lens f [1].

DNA – gold nanoparticle dynamics studied with in-liquid TEM (B) Examples of multimerization [2].

To cite this abstract:

Stephanie Manz, Sercan Keskin, Stephanie Besztejan, Benno Zeitler, Sana Azim, Guenther Kassier, Robert Buecker, Deybith Venegas-Rojas, Svenja Riekeberg, Dongfang Zhang, Albert Casandruc, Rolf A. Loch, Yinpeng Zhong, Hossein Delsim-Hashemi, Sascha W. Epp, Klaus Floettmann, Hoc Khiem Trieu, Andrea Rentmeister, R. J. Dwayne Miller; Dynamic-Transmission Electron Microscopy at the Relativistic Electron Gun for Atomic Exploration (REGAE) for live cell imaging. The 16th European Microscopy Congress, Lyon, France. https://emc-proceedings.com/abstract/dynamic-transmission-electron-microscopy-at-the-relativistic-electron-gun-for-atomic-exploration-regae-for-live-cell-imaging/. Accessed: July 13, 2020

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