Cellular organization depends on the cytoskeleton, a mechanical network of biopolymers that controls cell shape and strength, as well as on motor proteins that can move over these biopolymers to deliver cargo to specific subcellular compartments. Nevertheless, the precise mechanisms that control cytoskeletal organization, the function and dynamics of different motor proteins, and the precise functions of subcellular positioning are still poorly understood. In my lecture, I will highlight novel probes and methodology for the super-resolution imaging of the cytoskeleton. These approaches allow us to better resolve cytoskeletal organization in dense cellular compartments, such as the axons and dendrites of neurons. These technologies hold great promises for exploring cellular organization in health and disease.
To cite this abstract:Lukas Kapitein; Dissecting the neuronal microtubule cytoskeleton using optical nanoscopy. The 16th European Microscopy Congress, Lyon, France. https://emc-proceedings.com/abstract/dissecting-the-neuronal-microtubule-cytoskeleton-using-optical-nanoscopy/. Accessed: December 14, 2019
EMC Abstracts - https://emc-proceedings.com/abstract/dissecting-the-neuronal-microtubule-cytoskeleton-using-optical-nanoscopy/