The considerable growth of nano-technologies and nano-sciences requires the implementation of very well adapted characterization tools and methods to combine both a nanometer resolution and a good preservation of the nano-objects. This point is absolutely critical when the nanostructures are made of hydrated and organic compounds. Cryo-electron microscopy (cryo-TEM) made from vitrified thin films has proved to be a method of choice for characterizing the hierarchy of supramolecular assemblies of proteins and hydroxy fatty acids with maximum resolution and minimal damage.
We aim here to present a selection of applications of cryo-TEM showing the characterization of dispersions of organic or hybrid organic/inorganic nanoparticles with a large range of sizes, shapes and composition (synthetic polymers, polysaccharides, proteins, lipids).
In particular, we will detail how cryo-TEM helped to elucidate the supramolecular self-assembling of fatty acids through a multiscale approach. The unique benefits as well as the limitations of cryo-TEM will be discussed and compared with conventional SEM and TEM techniques as well as with liquid AFM.
Gaillard C. and Douliez JP. Cryo-TEM and AFM for the characterization of vesicle-like nanoparticle dispersions and self-assembled supramolecular fatty-acid-based structures: a few examples, in: A. Méndez-Vilas. (Ed.) Current microscopy contributions to advances in science and technology. 2012, v. 5, 912-922
F. Dutertre, C. Gaillard, C. Chassenieux, T. Nicolai, Branched Wormlike Micelles Formed by Self-Assembled Comblike Amphiphilic Copolyelectrolytes, Macromolecules, 2015; 48(20): DOI:10.1021/acs.macromol.5b01503
H.T.P. Nguyen, E. Allard-Vannier, C. Gaillard, I. Eddaoudi, L. Miloudi, M. Sourcé, I. Chourpa, E. Munnier “On the interaction of alginate-based core-shell nanocarriers with keratinocytes in vitro” Colloids and Surfaces B: Biointerfaces, 2016, accepté
Fameau, A.-L.; Saint-Jalmes, A.; Cousin, F.; Houinsou Houssou, B.; Novales, B.; Navailles, L.; Nallet, F.; Gaillard, C.; Boué, F.; Douliez, J.-P., 2011. Smart Foams: Switching Reversibly between Ultrastable and Unstable Foams. Angewandte Chemie International Edition, 50 (36): 8264-8269.
Douliez J.P. and Gaillard C., Self-assembly of fatty acids: from foams to protocell vesicles, New Journal of Chemistry, 2014, 38, 5142-5148
To cite this abstract:Cédric Gaillard; Cryo-electron microscopy from vitreous thin films in complement to AFM for understanding the multiscale supramolecular assemblies: a few examples.. The 16th European Microscopy Congress, Lyon, France. https://emc-proceedings.com/abstract/cryo-electron-microscopy-from-vitreous-thin-films-in-complement-to-afm-for-understanding-the-multiscale-supramolecular-assemblies-a-few-examples/. Accessed: December 1, 2022
EMC Abstracts - https://emc-proceedings.com/abstract/cryo-electron-microscopy-from-vitreous-thin-films-in-complement-to-afm-for-understanding-the-multiscale-supramolecular-assemblies-a-few-examples/