Nanoparticles are of great interest for their use in biomedical applications like imaging and drug delivery. They are used as delivery vesicles to carry drugs to cells, while protecting them from degradation and allowing a targeted delivery. It is important to understand the interaction of nanoparticles with the biological systems, in order to make these processes more efficient. Upon injection of nanoparticles inside the blood, there is a competition of different biological molecules to adsorb on the surface of the nanoparticles. Numerous proteins are present in the plasma at high concentrations and interact with the nanomaterial surface forming a cloud of proteins known as the “protein corona”. The protein corona alters the size and composition of a nanomaterial, giving it an identity which is not the same as its synthetic identity . The physical parameters of nanoparticles could affect the composition of protein corona, which is dynamic as the proteins are exchanging and the composition is evolving. This interface can be divided into the “hard” and the “soft” corona, depending on the binding strength and exchange rates of the proteins associated with the nanomaterial surface. The proteins with high binding affinities that are tightly bound to the nanoparticles form the “hard” protein corona and the ones that are loosely bound, have high exchange rates and can be easily replaced, form the “soft” corona. The cell or organ sees the nanoparticle-protein complexes when interacting with a nanoparticle dispersed in a biological medium. That means that the corona defines the biological identity of nanoparticles and influences the cytotoxicity and endocytosis. Due to the complexity of the protein corona, it has been challenging to characterize. Approaching the project with microscopy techniques will answer critical questions about the structure of nanoparticles and their interactions with the biological systems, which can be used to make biomedical applications more efficient.
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To cite this abstract:Maria Kokkinopoulou, Johanna Simon, Volker Mailaender , Ingo Lieberwirth , Katharina Landfester; Characterizing the protein corona of polystyrene nanoparticles. The 16th European Microscopy Congress, Lyon, France. https://emc-proceedings.com/abstract/characterizing-the-protein-corona-of-polystyrene-nanoparticles/. Accessed: May 24, 2019
EMC Abstracts - https://emc-proceedings.com/abstract/characterizing-the-protein-corona-of-polystyrene-nanoparticles/