EMC Abstracts

Official abstracts site for the European Microscopy Congress

MENU 
  • Home
  • Meetings Archive
    • The 16th European Microscopy Congress 2016
  • Keyword Index
  • Your Favorites
    • Favorites
    • Login
    • Register
    • View and Print All Favorites
    • Clear all your favorites
  • Advanced Search

3D investigation of a PS/ABS polymer using different microscopy techniques

Abstract number: 7044

Session Code: IM01-157

DOI: 10.1002/9783527808465.EMC2016.7044

Meeting: The 16th European Microscopy Congress 2016

Session: Instrumentation and Methods

Topic: Tomography and Multidimensional microscopy

Presentation Form: Poster

Corresponding Email: Sylvie.Descartes@insa-lyon.fr

X. Jaurand (1), E. Errazuriz (2), S. Reynaud (3), Th. Douillard (4), F. Dalmas (4), S. Descartes (5), F. Simonet (6), M. Mondon (7), I. Anselme-Bertrand (8)

1. Lyon BioImages, CTµ, Univ Lyon, UCB Lyon 1, Lyon, France 2. CIQLE, Univ Lyon, UCB Lyon 1, Lyon, France 3. LHC, UMR 5516, Univ Lyon, CNRS, Université J. Monnet, Saint-Etienne, France 4. MATEIS, UMR 5510, Univ Lyon, CNRS, INSA-Lyon, Lyon, France 5. LaMCoS UMR 5259, Univ Lyon, CNRS, INSA-Lyon, Lyon, France 6. IRCELyon, UMR 5256 , Univ Lyon, CNRS, UCB Lyon 1, Lyon, France 7. LGF, UMR 5307, Univ Lyon, CNRS, EMSE, Saint-Etienne, France 8. Faculté de médecine, Univ Lyon, Université J. Monnet, Saint-Etienne, France

Keywords: 3D imaging, FIB, polymer, SEM, Tomography

This study is developed in the framework of RéCaMiA, a French regional (Rhône-Alpes / Auvergne) microscopy network, which offers facilities through a panel of microscopes such as SEM, TEM, FIB, AFM, Confocal.

The objective of the study is to compare results obtained with different microscopy techniques currently employed in three-dimensional imaging, but it focuses more specifically on polymers [1-3]. For the purpose a blend of polystyrene / acrylonitrile butadiene styrene (PS/ABS) is chosen. This material allows to obtain good contrast in SEM or TEM; microstructural features exist at the submicronic and nano scales; and its glass transition temperature Tg is above 50°C. Two samples are stained by contact with a 4% osmium tetraoxide (OsO4) water solution to reveal contrasts. 2 FIB–SEM (FEI Helios600, Zeiss NVisio40 dual beam), 2 TEM (JEOL JEM 1400, JEOL 2100F), 2 SEM (FEI ESEM XL30, FEI Quanta250) are used to study the 3D structure of the polymer. This study develops a multiscale approach. The tools used require 2 different procedures to get a 3D image: (a) to acquire a stack of individual imaging planes and to transform it into the corresponding 3D data volume or (b) to perform electron tomography. In the first case individual images are obtained through a slice and view approach in FIB, or through individual observations, in SEM or TEM, of several thin foils prepared by ultramicrotomy method at room temperature (serial sectioning). Backscattered electron images are collected. The 3D reconstructions of the polymer obtained by the different tools are compared each other. Whatever the approach used, the morphology “salami” shape is observed in the structure of the polymer. Structure parameters, such as size distribution of the nodules and the anisotropy coefficient, are extracted and compared. Different test parameters effects are investigated, the preparation of the sample (compression of the microstructure more or less), the voltage, the spacing between the individual imaging planes, etc. The balance between the reconstructed volume size, the voxel resolution, the total time to get the 3D data volume, the advantages and limitations of the methods used in this multiscale approach will also be discussed.

[1] M. Kato et al., Journal of Polymer Science: Part B: Polymer Physics, Vol. 45, 677–683 (2007)
[2] S. Sinha Ray, Polymer 51 (2010) 3966-3970
[3] P. Jornsanoh et al., Ultramicroscopy 111(2011)1247–1254

Acknowledgements            

Thanks are due to CNRS for its financial support. Thanks are due to CLYM (Consortium Lyon – St-Etienne de Microscopie, www.clym.fr) funded by the Region Rhône-Alpes, the CNRS and the ‘GrandLyon’, for the access to the Zeiss NVisio40 and XL30; and also to Manutech-USD for the access to the FEI Helios600.

Figures:

Fig. 1. SEM images (serial sectioning) and 3D reconstruction (volume of 7x9x0.8 µm3)

Fig.2. 3D reconstruction volume obtained by slice and view in FIB-SEM

To cite this abstract:

X. Jaurand , E. Errazuriz , S. Reynaud , Th. Douillard , F. Dalmas , S. Descartes , F. Simonet , M. Mondon , I. Anselme-Bertrand ; 3D investigation of a PS/ABS polymer using different microscopy techniques. The 16th European Microscopy Congress, Lyon, France. https://emc-proceedings.com/abstract/3d-investigation-of-a-psabs-polymer-using-different-microscopy-techniques/. Accessed: September 21, 2023
  • Tweet
  • Email
  • Print
Save to PDF

« Back to The 16th European Microscopy Congress 2016

EMC Abstracts - https://emc-proceedings.com/abstract/3d-investigation-of-a-psabs-polymer-using-different-microscopy-techniques/

Most Viewed Abstracts

  • mScarlet, a novel high quantum yield (71%) monomeric red fluorescent protein with enhanced properties for FRET- and super resolution microscopy
  • 3D structure and chemical composition reconstructed simultaneously from HAADF-STEM images and EDS-STEM maps
  • Layer specific optical band gap measurement at nanoscale in MoS2 and ReS2 van der Waals compounds by high resolution electron energy loss spectroscopy
  • Pixelated STEM detectors: opportunities and challenges
  • Developments in unconventional dark field TEM for characterising nanocatalyst systems

Your Favorites

You can save and print a list of your favorite abstracts by clicking the “Favorite” button at the bottom of any abstract. View your favorites »

Visit Our Partner Sites

The 16th European Microscopy Congress

The official web site of the 16th European Microscopy Congress.

European Microscopy Society

European Microscopy Society logoThe European Microscopy Society (EMS) is committed to promoting the use and the quality of advanced microscopy in all its aspects in Europe.

International Federation of Societies for Microscopy

International Federation of Societies for Microscopy logoThe IFSM aims to contribute to the advancement of microscopy in all its aspects.

Société Française des Microscopies

Société Française des MicroscopiesThe Sfµ is a multidisciplinary society which aims to improve and spread the knowledge about Microscopy.

Connect with us

Imaging & Microscopy
Official Media Partner of the European Microscopy Society.

  • Help & Support
  • About Us
  • Cookie Preferences
  • Cookies & Privacy
  • Wiley Job Network
  • Terms & Conditions
  • Advertisers & Agents
Copyright © 2023 John Wiley & Sons, Inc. All Rights Reserved.
Wiley